Upon completion of the DSS, predictions will be evaluated in a series of field tests. The new system will then be used to predict the future outcomes of diverse management strategies. Inoculum from a distant source will be a new component of the system. We will modify the model to include the effects of new fungicides, and forecast weather. A currently existing late blight simulation model (Bruhn and Fry 1981) will serve as the basis for the DSS. Specific tagged lesions and we will then harvest sporangia from treated and untreated lesion to compare effects of fungicides on sporulation. We will measure growth rates of treated lesions in the field by taking digital images of Recently available fungicides have physical properties different from standard protectant fungicides and different from other systemic fungicides. c) Determine the epidemiological/biological effect of fungicides on pathogen development. We will modify the simulator to enable the ready incorporation of forecast weather. A second major limitation to current forecasts is the absence of consideration of forecast weather. We will quantify numbers of lesions and numbers of sporangia in a known source and then measure the concentration of sporangia that escape from the source. Thus we have initiated an investigation of this relationship. The quantitative relationship between source strength and airborne concentration of sporangia is completely unknown. One major limitation is ignorance of the availability of pathogen. There are several limitations to current forecast systems. The level of field resistance will be quantified in relation to the effect of a protectant fungicide applied regularly. ![]() Small plots of these plants will be inoculated under field conditions that favor late blight at Freeville, NY (near Ithaca) as has been described previously (Fry 1978). Cultivars, breeding lines, wild species, mutants, transgenic individuals, and segregating progeny of potatoes and tomatoes will be assayed for their field resistance to locally important strains of P. The experiments and computer simulation analyses will occur in Ithaca using labs and a computer simulation model already constructed and validated. The equipment/facilities/field plots for the field investigations are in place and have previously supported field experiments with potato and tomato late blight. Project Methods All of the procedures identified below will occur at Cornell University - either in field plots near Ithaca or in laboratories in Ithaca.
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